Seminar of André Stiel, Institute of Biological and Medical Imaging - Helmholtz Munich, Institute for Biophysics and Physical Biochemistry - University of Regensburg
16:00pm, Ecole Polytechnique, Salle à préciser
André C. Stiel
Web: Stiel-lab.de
Sense and switch across the scales - photoswitchable proteins for super-resolution and whole animal imaging - focus on Bacteriophytochromes.
Photoswitching proteins of the green fluorescent protein (GFP)-like family have found applications in super-resolution fluorescence imaging and beyond. In recent years, native photoswitching Bacteriophytochromes (BPhPs) have gained attention as labels in photo- / optoacoustic imaging. The development of BphPs likely follows a similar trajectory as their GFP-like counterparts, involving engineering their capabilities for imaging or constructing genetically encoded sensors. In all cases, a tight interplay of directed-evolution strategies and structural and mechanistic understanding is crucial. I will present our recent advancements in BphP engineering and understanding, as well as their applications in imaging.