Seminar by Martin WEIK, Institut de Biologie Structurale, CEA Grenoble
11AM, Amphi CURIE, Ecole Polytechnique
Watching proteins at work by kinetic X-ray crystallography
Kinetic X-ray crystallography permits the structural characterization of macromolecular conformational changes along a reaction pathway at the atomic level of spatial resolution. After triggering the biological reaction within a macromolecular crystal, functionally relevant conformational changes are either arrested by flash-cooling the crystal, allowing characterization of the structure by conventional cryo-crystallography (intermediate trapping), or followed in real time by time-resolved crystallography at room temperature. The temporal resolution of the latter is limited to 100 ps if carried out in the form of Laue crystallography at synchrotrons. The advent of X-ray free electron lasers (XFELs) has pushed the resolution to the sub-ps regime, allowing ultrafast changes to be studied by time-resolved serial femtosecond crystallography. After reviewing the current status of the field, we will illustrate the time-resolved crystallography approach with the study of photoisomerization of the chromophore in a reversibly photoswitchable fluorescent protein. We will indicate how information on ultrafast structural dynamics can be used to rationally modify photoswitchable fluorescent proteins for applications in biological imaging.
Contact : Marten Vos
marten.vos at polytechnique.edu