Publications

2006

• A filtering procedure for systematic removal of pump-perturbed polarization artifacts
  
  • Polack Thomas
  Optics Express, Optical Society of America - OSA Publishing, 2006, 14, pp.5823.
• Chiroptical effects in the second harmonic generation from collagens I and IV: applications in nonlinear microscopy
  
  • Pena Ana-Maria
  • Boulesteix Thierry
  • Dartigalongue Thibault
  • Strupler Mathias
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  Nonlinear optics, quantum optics, Old City Pub., 2006, 35 (1-3), pp.235-240. An emerging application of multiphoton microscopy is the observation of unstained intact biological tissues based on intrinsic sources of nonlinear signals. However, reliable biomedical imaging requires a thorough analysis of these endogenous signals. Looking at skin biopsies, we focused on 2-Photon Excited Fluorescence (2PEF) arising from cytokeratins in the epidermis, and second harmonic generation (SHG) from collagen fibers in the dermis. We determined the 2PEF excitation spectrum and action cross section of purified keratins from human epidermis, and obtained a good agreement with in situ measurements. In order to analyze the role of chirality and collagen type in SHG signal, we performed polarization-resolved surface SHG experiments on thin films of collagens I and IV molecules. We observed that collagen I and IV molecules exhibit comparable SHG signals, except for the chiral contributions which are absent for collagen IV and represent typically 50% of the signal for collagen I. We concluded that the large collagen I SHG efficiency is dominated by coherent effects due to the high density and quasi-crystalline order in collagen fibrils, whereas the lack of any alignment within the collagen IV molecules explains the absence of signal from this collagen type in SHG microscopy.
• Observer les neurones grâce aux ondes térahertz
  
  • Gallot Guilhem
  La Recherche, Sciences et avenir, 2006, 397, pp.26. Des chercheurs de l'École polytechnique ont développé un dispositif d'imagerie des neurones, fondée sur l'utilisation d'ondes térahertz [1]. Ils observent ainsi les mouvements d'ions et d'eau à l'intérieur et à proximité des neurones.
• Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity
  
  • Hunt D.E.
  • Klepac-Ceraj V.
  • Acinas S.G.
  • Bertilsson S.
  • Gautier Christian
  • Polz M.F.
  Applied and Environmental Microbiology, American Society for Microbiology, 2006, 72 (3), pp.2221. The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample. Copyright Cop. 2006, American Society for Microbiology. All Rights Reserved. (10.1128/AEM.72.3.2221-2225.2006)
  DOI : 10.1128/AEM.72.3.2221-2225.2006
• Micrometer scale ex vivo multiphoton imaging of unstained arterial wall structure
  
  • Boulesteix Thierry
  • Pena Ana-Maria
  • Pagès Nicole
  • Godeau G.
  • Sauviat Martin-Pierre
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  Cytometry Part A, Wiley, 2006, 69A (1), pp.20-26. We characterize the application of multiphoton microscopy to the observation of the extracellular matrix of fresh unstained vessels. Combined two-photon-excited fluorescence (2PEF) and second harmonic generation (SHG) imaging of large arteries reveals the architecture of elastin and collagen fibers in the vessel wall with remarkable specificity. We present elastin/collagen imaging in unstained rat vessels at both micrometer and whole vessel scales, and we characterize the optical properties of rat carotid artery and aorta walls. We apply this method to evidence deleterious effects of residual doses of a pesticide on the vessel wall. This study illustrates the potential of 2PEF/SHG microscopy for pharmacological studies in unlabeled arteries. © 2005 Wiley-Liss, Inc. (10.1002/cyto.a.20196)
  DOI : 10.1002/cyto.a.20196
• Role of Heme Iron Coordination and Protein Structure in the Dynamics and Geminate Rebinding of Nitric Oxide to H93G Myoglobin : Implications for NO-Sensors
  
  • Négrerie Michel
  • Kruglik Sergei
  • Lambry Jean-Christophe
  • Vos Marten H.
  • Martin Jean-Louis
  • Franzen Stefan
  Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281, pp.10389-10398.
• Molécules d'origine naturelle et nouveaux médicaments anticancéreux [Molecules of natural origin and new anticancer drugs]
  
  • Pages N.
  • Goudey-Perriere Françoise
  • Sauviat Martin-Pierre
  • Benoit Evelyne
  • Chamorro G.
  , 2006, pp.89-98.
• Terahertz achromatic quarter-wave plate
  
  • Masson Jean-Baptiste
  • Gallot Guilhem
  Optics Letters, Optical Society of America - OSA Publishing, 2006, 31 (2), pp.265. Phase retardera usually present a strong frequency dependence. We discuss the design and characterization of a terahertz achromatic quarter-wave plate. This wave plate is made from six birefringent quartz plates precisely designed and stacked together. Phase retardation has been measured over the whole terahertz range by terahertz polarimetry. This achromatic wave plate demonstrates a huge frequency bandwidth (?max/?min ˜ 7), and therefore can be applied to terahertz time domain spectroscopy and polarimetry. Cop. 2006 Optical Society of America. (10.1364/OL.31.000265)
  DOI : 10.1364/OL.31.000265
• Functional implications of the propionate 7 Arginine 220 interaction in the FixLH oxygen sensor from Bradyrhizobium japonicum
  
  • Balland Véronique
  • Bouzhir-Sima Latifa
  • Anxolabéhère-Mallart E.
  • Boussac A.
  • Vos Marten H.
  • Liebl Ursula
  • Mattioli T.
  Biochemistry, American Chemical Society, 2006, 45, pp.2072-2084.
• Role of distal arginine in early sensing intermediates in the heme domain of the oxygen sensor FixL
  
  • Jasaitis Audrius
  • Hola Klara
  • Bouzhir-Sima Latifa
  • Lambry Jean-Christophe
  • Balland Véronique
  • Vos Marten H.
  • Liebl Ursula
  Biochemistry, American Chemical Society, 2006, 45, pp.6018-6026.
• Nouvelle approche des fibroses par microscopie multiphotonique avec génération de second harmonique [New approach of fibrosis by multiphoton microscopy with second harmonic generation]
  
  • Hernest Monica
  • Pena Ana-Maria
  • Strupler Mathias
  • Beaurepaire Emmanuel
  • Martin Jean-Louis
  • Schanne-Klein Marie-Claire
  • Tharaux Pierre-Louis
  Médecine/Sciences, EDP Sciences, 2006, 22 (10), pp.820-821. La fibrose est une réponse adaptative pathologique qui détruit non spécifiquement les tissus. Il s'agit d'un processus universel de réparation des tissus qui survient en réaction à de nombreux types d'agressions telles les contraintes mécaniques, les brûlures, les radiations ionisantes, l'ischémie, l'inflammation. Ces agressions concourent de manière intriquée à la physiopathologie des maladies infectieuses, tumorales ou auto-immunes, de l'hypertension artérielle et des maladies cardio-vasculaires. Le terme de fibrose décrit précisément l'accumulation nouvelle de protéines de la matrice extra-cellulaire selon un arrangement spatial fibrillaire caractéristique. Il s'agit essentiellement de molécules de collagènes de type I et III (voire de type II, V ou XI) synthétisées sous forme de triples hélices elles-mêmes assemblées en fibrilles par les cellules fibroblastiques. L'apparition des collagènes fibrillaires marque un changement qualitatif et quantitatif de composition des collagènes des tissus. Réciproquement, ces changements de la matrice extracellulaire influencent le phénotype des cellules qui y résident. Ainsi dans le rein normal, le collagène de type I n'existe que dans l'adventice artériel. Son apparition au sein des autres structures de cet organe marque une fibrose tubulo-interstitielle qui constitue le meilleur marqueur pronostic défavorable d'une évolution vers l'insuffisance rénale terminale, et ce quelle que soit la maladie causale. Ainsi, comme lors des fibroses compliquant les hépatopathies et les pneumopathies chroniques, les séquelles de brûlures ou d'abrasions cutanées-muqueuses ou encore le remodelage cardiaque et vasculaire, le réarrangement de la géométrie de la matrice extracellulaire altère l'organisation fonctionnelle du tissu considéré. De ce fait, ce processus de réparation a des effets fonctionnels délétères qui constituent un enjeu médical majeur. Les fibrilles de collagène ont des capacités d'auto-assemblage qui sont aussi catalysées et stabilisées ou au contraire empêchées par les enzymes de la matrice extracellulaire. Le développement de la fibrose ou sa régression dépend donc ainsi du bilan des équilibres biologiques de ces mécanismes. Il est donc crucial de caractériser les changements extracellulaires et cellulaires qui font du restutio ad integrum de l'architecture et de la fonction tissulaire un défi biomédical. (10.1051/medsci/20062210820)
  DOI : 10.1051/medsci/20062210820
• Mechanisms involved in the swelling of erythrocytes caused by Pacific and Caribbean ciguatoxins
  
  • Sauviat Martin-Pierre
  • Boydron-Le Garrec Raphaële
  • Masson Jean-Baptiste
  • Lewis Richard L.
  • Vernoux Jean-Paul
  • Molgó Jordi
  • Laurent Dominique
  • Benoit Evelyne
  Blood Cells, Molecules and Diseases, Elsevier, 2006, 36(1), pp.1-9. The mechanisms underlying the swelling of frog red blood cells (RBC), induced by Pacific (P-CTX-1) and Caribbean (C-CTX-1) ciguatoxins (CTXs), were investigated by measuring the length, width and surface of their elliptic shape. P-CTX-1 (0.5 to 5 nM) and C-CTX-1 (1 nM) induced RBC swelling within 60 min. The CTXs-induced RBC swelling was blocked by apamin (1 μM) and by Sr2+ (1 mM). P-CTX-1-induced RBC swelling was prevented and inhibited by H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (27 μM), an inhibitor of soluble guanylate cyclase (sGC), and NOS blockade by NG methyl-l-arginine (l-NMA; 10 μM). Cytochalasin D (cytD, 10 μM) increased RBC surface and mimicked CTX effect but did not prevent the P-CTX-1-induced l-NMA-sensitive extra increase. Calculations revealed that P-CTX-1 and cytD increase RBC total surface envelop and volume. These data strongly suggest that the molecular mechanisms underlying CTXs-induced RBC swelling involve the NO pathway by an activation of the inducible NOS, leading to sGC activation which modulates intracellular cGMP and regulates L-type Ca2+ channels. The resulting increase in intracellular Ca2+ content, in turn, disrupts the actin cytoskeleton, which causes a water influx and triggers a Ca2+-activated K+ current through SK2 isoform channels. (10.1016/j.bcmd.2005.10.007)
  DOI : 10.1016/j.bcmd.2005.10.007
• Nonlinear microscopy using Second Harmonic Generation from myosin filaments and fibrillar collagens
  
  • Pena Ana-Maria
  • Strupler Mathias
  • Boulesteix Thierry
  • Fabre Aude
  • Hernest Monica
  • Tharaux Pierre-Louis
  • Crestani Bruno
  • Martin Jean-Louis
  • Sauviat Martin-Pierre
  • Beaurepaire Emmanuel
  • Schanne-Klein Marie-Claire
  , 2006.
• Photoinduced electron transfer from a novel nanotrigger addressed to the NADPH site within the endothelial NO-synthase to the flavin moieties of the protein
  
  • Beaumont Edward
  • Robin Anne-Claire
  • Berka Vladimir
  • Tsai Ah-Lim
  • Blanchard-Desce Mireille
  • Lambry Jean-Christophe
  • Slama-Schwok Anny
  Nitric Oxide: Biology and Chemistry, Elsevier, 2006, 14 (4), pp.14-15. We developed a new selective molecular tool to trigger enzymatic activity in a synchronous manner and monitor the sequence of the kinetic events by ultra-fast transient spectroscopy. Our approach is based on a synthetic nanotrigger addressing a selected site within proteins, namely the conserved NADPH binding site common to many enzymes involved in bioreductive processes [1]. The nanotrigger combines a "docking" subunit responsible for the recognition of NADPH sites within proteins and a "chromophoric" subunit responsive to light excitation and able to transfer electrons to the flavin moieties of proteins. We present the first spectroscopic data on such a nanotrigger [1] in the presence of the reductase domain of the endothelial nitric oxide synthase eNOSred. (10.1016/j.niox.2006.04.050)
  DOI : 10.1016/j.niox.2006.04.050
• Role of the middle residue in the triple tryptophan electron transfer chain of DNA photolyase: ultrafast spectroscopy of a Trp→Phe mutant
  
  • Lukacs Andras
  • Eker A.P.M.
  • Byrdin M.
  • Villette Sandrine
  • Pan J.
  • Brettel K.
  • Vos Marten H.
  Journal of Physical Chemistry B, American Chemical Society, 2006, 110 (32), pp.15654-15658.
• Endothelial nitric oxide synthase reduces nitrite anions to NO under anoxia
  
  • Gautier Clément
  • van Faassen E.
  • Mikula I.
  • Martasek P.
  • Slama-Schwok Anny
  Biochemical and Biophysical Research Communications, Elsevier, 2006, 341 (3), pp.816-821. In this work, we demonstrate that endothelial nitric oxide synthase is capable of anoxic reduction of nitrite anions to nitric oxide at physiological pH by absorption and EPR spectroscopy and electrochemical measurements. The nitrite reduction is achieved at the oxygenase domain of the protein and proceeds even in the absence of the tetrahydrobiopterin cofactor. The nitrite pathway increases by sixfold the NO production with respect to the regular arginine pathway under hypoxia, which is largely blocked. Therefore, basal levels of NO release could be sustained by anoxic nitrite reduction. The reaction suggests a new pathway for fast NO delivery under hypoxia, precisely when the vasodilating properties of nitric oxide are most needed. (c) 2006 Elsevier Inc. All rights reserved. (10.1016/j.bbrc.2006.01.031)
  DOI : 10.1016/j.bbrc.2006.01.031
• Studying intra-protein signaling intermediates in the heme-based oxygen sensor protein FixL using sub-picosecond transient Raman spectroscopy
  
  • Vos Marten H.
  , 2006, pp.oral.
• Second harmonic generation by collagens I and IV: chiroptical and structural effects
  
  • Pena Ana-Maria
  • Boulesteix Thierry
  • Dartigalongue Thibault
  • Schanne-Klein Marie-Claire
  , 2006.
• Dynamique interne dans les hémoprotéines vue par spectroscopie ultrarapide
  
  • Vos Marten H.
  , 2006.
• Manipulation of morphogenetic movements in live Drosophila embryos using femtosecond pulses
  
  • Supatto Willy
  • Débarre Delphine
  • Martin Jean-Louis
  • Schanne-Klein Marie-Claire
  • Farge Emmanuel
  • Beaurepaire Emmanuel
  , 2006.
• Parametric cascade downconverter for intense ultrafast mid-infrared generation beyond the Manley-Rowe limit
  
  • Fraser James M.
  • Ventalon Cathie
  Applied optics, Optical Society of America, 2006, 45 (17), pp.4109-4113. We propose a scheme to generate intense, ultrafast mid-infrared pulses with conversion efficiencies exceeding the upper bound for single-stage difference-frequency mixing as predicted by the Manley-Rowe relations. Finite-element fast Fourier transform simulations of the mixing process show that the parametric cascade downconverter generates 1.7 times more photons (at 10 μm ) than in the initial pump pulse (center wavelength of 1.48 μm , duration of 130 fs , and pulse energy of 50 μJ ), with negligible pulse spatial and temporal distortion. © 2006 Optical Society of America (10.1364/AO.45.004109)
  DOI : 10.1364/AO.45.004109
• Measuring the dynamics of circular dichroism in a pump-probe experiment with a Babinet-Soleil compensator
  
  • Niezborala Claire
  • Hache François
  Journal of the Optical Society of America B, Optical Society of America, 2006, 23 (11), pp.2418. Circular dichroism contains rich information on the conformation of molecules and, in particular, of biomolecules, and measuring its variation in a pump-probe experiment is very promising but also very challenging. We propose a new technique to measure pump-induced variation of the circular dichroism, which is based on the measurement of the probe ellipticity and its variation with the pump. This technique has the advantage that it does not require modulation of the probe polarization, which allows modulation of the pump intensity. We show theoretically and demonstrate experimentally that this technique is very sensitive and user friendly. We also show that it can be used to measure pump-induced change in the optical rotation, allowing for a complete characterization of pump-induced optical activity. Cop. 2006 Optical Society of America. (10.1364/JOSAB.23.002418)
  DOI : 10.1364/JOSAB.23.002418
• Coupling between surface plasmons in subwavelength hole arrays
  
  • Masson Jean-Baptiste
  • Gallot Guilhem
  Physical Review B: Condensed Matter and Materials Physics (1998-2015), American Physical Society, 2006, 73, pp.121401.
• Ionic contrast terahertz near field imaging of axonal water fluxes
  
  • Masson Jean-Baptiste
  • Sauviat Martin-Pierre
  • Martin Jean-Louis
  • Gallot Guilhem
  Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2006, 103 (13), pp.4808-4812.
• Hydrophobic distal pocket affects NO-heme geminate recombination dynamics in dehaloperoxidase and H64V myoglobin
  
  • Franzen S.
  • Jasaitis Audrius
  • Belyea J.
  • Brewer S.
  • Casey Romain
  • Macfarlane A.
  • Martin Jean-Louis
  • Stanley R.
  • Vos Marten H.
  Journal of Physical Chemistry B, American Chemical Society, 2006, 110 (29), pp.14483. The recombination dynamics of NO with dehaloperoxidase ( DHP) from Amphitrite ornata following photolysis were measured by femtosecond time- resolved absorption spectroscopy. Singular value decomposition ( SVD) analysis reveals two important basis spectra. The first SVD basis spectrum reports on the population of photolyzed NO molecules and has the appearance of the equilibrium difference spectrum between the deoxy and NO forms of DHP. The first basis time course has two kinetic components with time constants of tau(11) approximate to 9 ps and tau(12) approximate to 50 ps that correspond to geminate recombination. The fast geminate process tau(11) arises from a contact pair with the heme iron in a bound state with S) 3/2 spin. The slow geminate process tau(12) corresponds to the recombination from a more remote docking site > 3 angstrom from the heme iron with the greater barrier corresponding to a S) 5/ 2 spin state. The second SVD basis spectrum represents a time- dependent Soret band shift indicative of heme photophysical processes and protein relaxation with time constants of tau(21) approximate to 3 ps and tau(22) approximate to 17 ps, respectively. A comparison between the more rapid rate constant of the slow geminate phase in DHP- NO and horse heart myoglobin ( HHMbNO) or sperm whale myoglobin ( SWMbNO) suggests that protein interactions with photolyzed NO are weaker in DHP than in the wild- type MbNOs, consistent with the hydrophobic distal pocket of DHP. The slower protein relaxation rate tau(22) in DHP- NO relative to HHMbNO implies less effective trapping in the docking site of the distal pocket and is consistent with a greater yield for the fast geminate process. The trends observed for DHP- NO also hold for the H64V mutant of SWMb ( H64V MbNO), consistent with a more hydrophobic distal pocket for that protein as well. We examine the influence of solution viscosity on NO recombination by varying the glycerol content in the range from 0% to 90% ( v/ v). The dominant effect of increasing viscosity is the increase of the rate of the slow geminate process, tau(12), coupled with a population decrease of the slow geminate component. Both phenomena are similar to the effect of viscosity on wild- type Mb due to slowing of protein relaxation resulting from an increased solution viscosity and protein surface dehydration. (10.1021/jp056790m)
  DOI : 10.1021/jp056790m