Laboratoire d'optique et biosciences
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Tutelle https://www.cnrs.fr/fr Tutelle https://www.polytechnique.edu/ Tutelle https://www.inserm.fr/
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DNA Replication in Archaea

 

How Archaea, the third domain of Life, replicate their DNA and maintain their genomes intact often under deleterious environmental conditions is also investigated by our team.

We have established the salt-loving archaea Haloferax volcanii as a unique model for studying archaeal DNA replication and investigate the function of the helicase/nuclease Hef in replication restart. By expressing replication proteins fused to the Green Fluorescent protein, and using fluorescence microscopy technics, we study their localization in living cells to better understand replication dynamics in these highly polyploidy archaeal cells.

 

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Selected publications:

  •  Lestini R, Collien Y, Olivier D, Olivier N, Myllykallio H. BrdU Incorporation and Labeling of Nascent DNA to Investigate Archaeal Replication Using Super-Resolution Imaging. Methods Mol Biol. 2022;2522:419-434. doi:10.1007/978-1-0716-2445-6_29. PMID: 36125768.

  • Cockram C, Thierry A, Gorlas A, Lestini R, Koszul R. Euryarchaeal genomes are folded into SMC-dependent loops and domains, but lack transcription-mediated compartmentalization. Mol Cell. 2021 Feb 4;81(3):459-472.e10. doi:10.1016/j.molcel.2020.12.013. Epub 2020 Dec 30. PMID: 33382984.

  • Delpech F, Collien Y, Mahou P, Beaurepaire E, Myllykallio H, Lestini R. Snapshots of archaeal DNA replication and repair in living cells using super-resolution imaging. Nucleic Acids Res. 2018 Nov 16;46(20):10757-10770. doi:10.1093/nar/gky829. PMID: 30212908; PMCID: PMC6237752.