Séminaire de Stéphanie BURY-MONE, I2BC, Gif-sur-Yvette
à 11h, Amphi CURIE, Ecole Polytechnique
Dynamics of Streptomyces genome architecture and genomic island expression
Streptomyces are characterized by a large linear chromosome divided into a central region harboring cores genes and two extremities enriched in poorly conserved sequences including genomic islands (GIs) encoding notably specialized metabolite biosynthetic gene clusters (SMBGCs) or prophages. The majority of these GIs remain transcriptionally silent over growth under lab conditions. We previously demonstrated in Streptomyces ambofaciens ATCC 23877 that this genetic compartmentalization correlates with chromosome architecture and gene expression during vegetative growth: The distal ribosomal RNA (rrn) operons delimit a highly structured and expressed region termed ‘central compartment’, presenting structural features distinct from those of the terminal compartments which are almost transcriptionally quiescent. This architecture is dynamic during cell growth. We thus further explored the link between chromosome architecture dynamics and GI expression. First, we analyzed chromosome dynamics in a stress condition associated with prophage and other mobile genetic elements activation. Second, we explored the impact of relocating an SMBGC from the terminal to the central compartments. Altogether, these results highlights the extent of chromosome architecture dynamics and its links with GI expression and Streptomyces physiology.
Keywords: Chromosome architecture; Genomic islands ; Spatial compartmentalization ; Phage ; SMBGC ; Transcription
Publications:
Lioy VS, Lorenzi JN, Najah S, Poinsignon T, Leh H, Saulnier C, Aigle B, Lautru S, Thibessard A, Lespinet O, Leblond P, Jaszczyszyn Y, Gorrichon K, Varoquaux N, Junier I, Boccard F, Pernodet JL, Bury-Moné S. Dynamics of the compartmentalized Streptomyces chromosome during metabolic differentiation. Nat Commun. 2021 doi: 10.1038/s41467-021-25462-1
Lorenzi JN, Thibessard A, Lioy VS, Boccard F, Leblond P, Pernodet JL, Bury- Moné S. Ribosomal RNA operons define a central functional compartment in the Streptomyces chromosome. Nucleic Acids Res. 2022 doi: 10.1093/nar/gkac1076
Jaffal H., Kortebi M., Misson P., Tavares P., Ouldali M., Leh H., Lautru S., Lioy V.S., Lecointe F., Bury-Moné S., “Phage-mediated dispersal of multicellular bacteria” BioRxiv (2023) - doi: https://doi.org/10.1101/2023.07.22.549817 - under revision for PloS Biology
Contact : Stéphane Skouloubris
stephane.skouloubris at universite-paris-saclay.fr