Pierre Mahou
Research Engineer, Ecole Polytechnique
Room: 84-20-17
Phone: (+33) 1 69 33 50 63
pierre.mahou@polytechnique.edu
ORCID ID : 0000-0003-4452-1011
Google scholar profile
ResearcherID: F-2089-2017
Current projects and responsibilities:
In charge of the imaging facility Morphoscope: Training and scientific counselling of microscope users, contribution to the research projects, maintenance and upgrade of commercial systems
In charge of the design and implementation of optical microscopes and contribution to the research projects in the Advanced microscopies & tissue physiology research group such as for: Large-scale multicolor microscopy, Two-photon Light-Sheet microscopy, Three-photon microscopy for deep-tissue imaging
Since 2015: 30 publications in high impact journals such as: Nature, Nature Methods, Nature Communications, PNAS, Light Science & Applications, Science Advances, Cell Stem Cell, h-index of 22
For more details see our group webpage: Advanced microscopies & tissue physiology
Co-supervision of PhD theses:
Since 2023: Solene Prudhomme (with E. Beaurepaire)
2019 - 2023: Hugo Blanc, "Large-volume color 2P microscopy for mouse brain imaging" (with E. Beaurepaire, defense 11/2023)
Background:
2015-Present: Research engineer at Laboratory for Optics and Biosciences (Ecole Polytechnique, France)
2013-2015: Post-doctoral researcher at Laser Analytics Group (University of Cambridge, UK)
2013: Post-doctoral researcher at Laboratory for Optics and Biosciences (Ecole Polytechnique, France)
2009-2012: PhD in Biophysics at Laboratory for Optics and Biosciences (Ecole Polytechnique, France),
Title: Nonlinear microscopy of biological tissues: multicolor excitation, Bessel beams and light sheet microscopy
Thesis Advisors: Dr. Emmanuel Beaurepaire and Dr. Delphine Débarre
Teaching and other tasks:
2023: President of the jury for the "Thesis Prize Pierre Favard" in Life Sciences
Since 2022: Board member of the "Société Française des Microscopies (Sfμ)"
Since 2020: Course "Multiphoton microscopy" at the "Atelier de Microscopie Confocale" (CNRS Formation Entreprises)
Since 2019: Coordinator of the course: "Impulsions Optiques Ultra-Brèves et Applications" in the M2 Quantum, Light, Materials and Nano Sciences (Université Paris Saclay)
Publications:
Full list on my Google Scholar profile
Preprints:
Spatial modeling of telomere intra-nuclear distribution reveals non-random organization that varies during cell cycle and depends on LAP2 and BAF, D. Keller, S. Stinus, D. Umlauf, E. Gourbeyre, E. Biot, N. Olivier, P. Mahou, E. Beaurepaire, P. Andrey, L. Crabbe, bioRχiv, (2022)
Selected publications:
Chromatically corrected multicolor multiphoton microscopy
H. Blanc, G. Kaddour, N. B. David, W. Supatto, J. Livet, E. Beaurepaire, P. Mahou
ACS Photonics (2023)
Fluorescence to measure light intensity
A. Lahlou, H. Sepasi Tehrani, I. Coghill, Y. Shpinov, M. Mandal, M.-A. Plamont, I. Aujard, Y. Niu, L. Nedbal, D. Lazar, P. Mahou, W. Supatto, E. Beaurepaire, I. Eisenmann, N. Desprat, V. Croquette, R. Jeanneret, T. Le Saux, L. Jullien,
Nature Methods (2023)
Apical size and deltaA expression predict adult neural stem cell decisions along lineage progression
L. Mancini, B. Guirao, S. Ortica, M. Labusch, F. Cheysson, V. Bonnet, M.-S. Phan, S. Herbert, P. Mahou, E. Menant, S. Bedu, J.-Y. Tinevez, C. Baroud, E. Beaurepaire, Y. Bellaiche, L. Bally-Cuif, N. Dray
Science Advances (2023)
Label-free imaging of red blood cells and oxygenation with color third-order sum-frequency generation microscopy
J. Ferrer Ortas, P. Mahou, S. Escot, C. Stringari, N. David, M. Négrerie, W. Supatto, N. Dray, and L. Bally-Cuif, E. Beaurepaire,
Light Science and Applications (2023)
Dynamic spatiotemporal coordination of neural stem cell fate decisions through local feedback in the adult vertebrate brain
N. Dray, L. Mancini, U. Binshtok, F. Cheysson, W. Supatto, P. Mahou, S. Bedu, S. Ortica, M. Krecsmarik, S. Herbert, J.-B. Masson, J.-Y. Tinevez, G. Lang, E. Beaurepaire, D. Sprinzak, L. Bally-Cuif,
Cell Stem Cell (2021)
Fast In Vivo Imaging of SHG Nanoprobes with Multiphoton Light-Sheet Microscopy
G. Malkinson, P. Mahou, E. Chaudan, T. Gacoin, A. Y Sonay, P. Pantazis, E. Beaurepaire, W. Supatto,
ACS Photonics (2020)
Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency
V. Maioli, A. Boniface, P. Mahou, J. Ferrer Ortas, L. Abdeladim, E. Beaurepaire, W. Supatto,
Biomedical Optics Express (2020)
Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
L. Abdeladim, K. S. Matho, S. Clavreul, P. Mahou, J.-M. Sintes, X. Solinas, I. Arganda-Carreras, S. G. Turney, J. W. Lichtman, A. Chessel, A.-P. Bemelmans, K. Loulier, W. Supatto, J. Livet, E. Beaurepaire,
Nature Communications (2019)
An actin-based viscoplastic lock ensures progressive body-axis elongation
A. Lardennois, G. Pásti, T. Ferraro, F. Llense, P. Mahou, J. Pontabry, D. Rodriguez, S. Kim, S. Ono, E. Beaurepaire, C. Gally, M. Labouesse,
Nature (2019)
Dual-color deep-tissue three-photon microscopy with a multiband infrared laser
K. Guesmi, L. Abdeladim, S. Tozer, P. Mahou, T. Kumamoto, K. Jurkus, P. Rigaud, K. Loulier, N. Dray, P. Georges, M. Hanna, J. Livet, W. Supatto, E. Beaurepaire, F. Druon
Light Science & Applications (2018)
Metrology of Multiphoton Microscopes Using Second Harmonic Generation Nanoprobes
P. Mahou, G. Malkinson, É. Chaudan, T. Gacoin, E. Beaurepaire, W. Supatto
Small (2017)
Multicolor two-photon light-sheet microscopy
P. Mahou, J. Vermot, E. Beaurepaire & W. Supatto
Nature Methods (2014)
Multicolor two-photon tissue imaging by wavelength mixing
P. Mahou, M.S. Zimmerley, K. Loulier, K.S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre & E. Beaurepaire
Nature Methods (2012)