Nonlinear polarimetry

Polarization-resolved multiphoton microscopy (nonlinear polarimetry) provides submicron information on organized samples.

We develop P-SHG to probe collagen macromolecular organization, and P-THG to probe ordered lipids and biominerals.

Related publications

Modeling and predicting second-harmonic generation from protein molecular structure, B. Asadipour, E. Beaurepaire, X. Zhang, A. Chessel, P. Mahou, W. Supatto, M.-C. Schanne-Klein, C. Stringari, Physical Review X (2024).
Kidney stone classification using multimodal multiphoton microscopy, M. Gleeson, J. Morizet, P. Mahou, M. Daudon, D. Bazin, C. Stringari, M.-C. Schanne-Klein, and E. Beaurepaire, ACS Photonics (2023).
Unveiling the lamellar structure of the human cornea over its full thickness using polarization-resolved SHG microscopy, C. Raoux, A. Chessel, P. Mahou, G. Latour, and M.-C. Schanne-Klein, Light: Science and Applications 12, 190 (2023).
Noninvasive quantitative assessment of collagen degradation in parchments by polarization-resolved SHG microscopy, M Schmeltz, L Robinet, S Heu-Thao, JM Sintes, C Teulon, G Ducourthial, P Mahou, MC Schanne-Klein, G Latour, Science Advances (2021)
Modeling nonlinear microscopy near index-mismatched interfaces, J Morizet, G Sartorello, N Dray, C Stringari, E Beaurepaire, N Olivier, Optica (2021)
Quantitative structural imaging of keratoconic corneas using polarization-resolved SHG microscopy, C Raoux, M Schmeltz, M Bied, M Alnawaiseh, U Hansen, G Latour, MC Schanne-Klein, Biomedical optics express (2021)
Circular dichroism second-harmonic generation microscopy probes the polarity distribution of collagen fibrils, M Schmeltz, C Teulon, M Pinsard, U Hansen, M Alnawaiseh, D Ghoubay, V Borderie, G Mosser, C Aimé, F Légaré, G Latour, MC Schanne-Klein, Optica (2020)
Fast In Vivo Imaging of SHG Nanoprobes with Multiphoton Light-Sheet Microscopy, G Malkinson, P Mahou, E Chaudan, T Gacoin, A Sonay, P Pantazis, E Beaurepaire, W Supatto, ACS photonics (2020)
High-speed polarization-resolved third-harmonic microscopy, J. Morizet, G. Ducourthial, W. Supatto, A. Boutillon, R. Legouis, M.-C. Schanne-Klein, C. Stringari, E. Beaurepaire, Optica 6 (3), 385-388 (2019).
Monitoring dynamic collagen reorganization during skin stretching with fast polarization‐resolved second harmonic generation imaging, G. Ducourthial, J.-S. Affagard, M. Schmeltz, X. Solinas, M. Lopez‐Poncelas, C. Bonod‐Bidaud, R. Rubio‐Amador, F. Ruggiero, J.‐M. Allain, E. Beaurepaire, M.‐C. Schanne‐Klein, J. Biophotonics e201800336 (2019).
Probing the 3D structure of cornea-like collagen liquid crystals with polarization-resolved SHG microscopy, C. Teulon, A. Tidu, F. Portier, G. Mosser, and M.-C. Schanne-Klein, Opt. Express 24, 16084 (2016).
Theoretical, numerical and experimental study of geometrical parameters that affect anisotropy measurements in polarization-resolved SHG microscopy, C. Teulon, I. Gusachenko, G. Latour, and M.-C. Schanne-Klein, Opt. Express 23, 9313-9328 (2015)
Determination of collagen fiber orientation in histological slides using Mueller microscopy and validation by second harmonic generation imaging, S. Bancelin, A. Nazac, B. H. Ibrahim, P. Dokladal, E. Decencière, B. Teig, H. Haddad, H. Fernandez, M.-C. Schanne-Klein, A. de Martino, Opt. Express 22, 22561 (2014)
Probing ordered lipid assemblies with polarized third-harmonic-generation microscopy, M. Zimmerley, P. Mahou, D. Débarre, M.-C. Schanne-Klein & E. Beaurepaire, Phys. Rev. X 3, 011002 (2013).
Numerical simulation of polarization-resolved second harmonic microscopy in birefringent media, I. Gusachenko and M.-C. Schanne-Klein, Phys. Rev. A 88, 053811 (2013)
Polarization-resolved second-harmonic microscopy in tendon upon mechanical stretching, I. Gusachenko, Y. G. Houssen, V. Tran, J.-M. Allain, M.-C. Schanne-Klein, Biophys. J. 102, 2220 (2012).
In vivo structural imaging of the cornea by polarization-resolved Second Harmonic microscopy, G. Latour, I. Gusachenko, L. Kowalczuk, I. Lamarre, M.-C. Schanne-Klein, Biomed. Opt. Express 3, 1 (2012).
Harmonic microscopy of isotropic and anisotropic microstructure of the human cornea, N. Olivier, F. Aptel, K. Plamann, M.-C. Schanne-Klein & E. Beaurepaire, Opt. Express 18(5), 5028-40 (2010).
Polarization-resolved Second Harmonic microscopy in thick anisotropic tissues, I. Gusachenko, G. Latour, M.-C. Schanne-Klein, Opt. Express 18, 19339 (2010)