Advanced microscopies and tissue physiology
Ongoing advances in optical imaging open novel possibilities for studying intact biological tissues with subcellular resolution. We develop experimental strategies for removing bottlenecks in experimental systems biology based on our expertise in nonlinear optics, tissue microscopy, image analysis, cell/developmental biology, and biophysics. Our activity consists of two complementary aspects (i) microscopy methods development including proof-of-principle studies, and (ii) studying embryonic development and tissue function using multidisciplinary approaches.
Methods: biophotonics, multiphoton microscopy, light-sheet, 2PEF, 3PEF, SHG, THG, FLIM, CARS, SIM, polarization, laser ablation, terahertz. Topics: embryogenesis, neurodevelopment, collagen, heart, cornea, extracellular matrix. Models: zebrafish, drosophila, mouse (ex vivo), tissue biopsies, cell cultures.
PEOPLE
• Emmanuel Beaurepaire (CNRS DR)
• Anatole Chessel (EP Asst Prof)
• Nicolas David (CNRS DR)
• Sophie Escot (INSERM CR)
• Guilhem Gallot (CNRS DR)
• Gaël Latour (UPSay Asst Prof)
• Pierre Mahou (EP IR)
• Karsten Plamann (ENSTA PR)
• Marie-Claire Schanne-Klein (CNRS DR)
• Chiara Stringari (CNRS CR)
• Willy Supatto (CNRS DR)
Postdocs
• Payvand Arjmand
• Matthew Gleeson
• Blandine Lordon
• Xiaotong Yuan
• Lei Zhu
• Vaky Abdelsayed
• Xue Bing
• Nuno Bras
• Matheus Cândia Araña
• Clément Caporal
• Stella Dees
• Amélie Elouin
• Giulia Galante
• Yara Hassanein
• Poncia Nyembo
• Solène Prudhomme
• Victor Struillou
Technical support
• Xavier Solinas - electronics
• Jean-Marc Sintes - mechanics
• Isabelle Lamarre-Jouenne - biotechnology
• Emilie Menant - zebrafish husbandry
Collaborators and Alumni • Group pictures
Work with us: Internships / PhD proposals / postdoc positions
PROJECTS
Avanced multiphoton microscopy
• Second harmonic generation (SHG) in skin and cornea
• Brain-wide multicolor 2P microscopy
• Two-photon light sheet microscopy (2P-SPIM)
• FLIM-based metabolic imaging
• Deep-tissue 3-photon microscopy
• Third harmonic generation (THG) in myelin, blood and biocrystals
• Nonlinear polarimetry
Other imaging methods
• Large-volume bioimage informatics
• Terahertz spectroscopy and imaging
• Adaptive optics & focus shaping
• Equipex Morphoscope / imaging facility
Embryonic and organ development
• Cell migration and morphogenesis in the early embryo
• Multiphoton microscopy of developing tissues
Biomaterials structure and function
• Advanced imaging of cultural heritage artefacts
• Transparency of the human cornea
Past projects
• Cilia imaging and left-right symmetry breaking
• Tissue micromanipulation and embryo biomechanics
• Nonlinear microscopy with broadband ultrashort pulses
